Different factors affect developmental competence and cryotolerance in in vitro produced bovine embryo.

Impact of in vitro fertilization by fresh and frozen semen on developmental competence and cryotolerance of buffalo embryos

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...

Effect of Culture System on Developmental Competence, Cryosurviva and DNA-Fragmentation of In Vitro Bovine Blastocysts

O-7: Improved In Vitro Development of Cloned Bovine Embryos Using S-Adenosylhomocysteine,A Non-Toxic Epigenetic

Background: Development of cloned bovine embryos. Materials and Methods: Oocytes collection,oocyte denudation, oocyte enucleation, nuclear transfer, electrofusion, reconstructed embryo activation, culture of reconstructed and IVF embryo,and treatment with SAH post fusion interval Treatment of reconstructed embryos with TSA for 12 hours after activation, preparation of somatic donor cells, donor...

In Vitro Development of Cattle Embryo as Affected By Glucose, Serum and EDTA

Purpose: The aim of this study was to evaluate the effect of different modifications of sequential synthetic oviductal_fluid (SOF) culture system on developmental competence of in vitro matured/fertilized cattle embryos. Materials and Methods: Bovine oocytes were matured and fertilized in vitro and then presumptive zygotes were randomly cultured for up to 9 days in different modifications of SO...

The Effect of Different Voltages and Durations on Developmental Rate of Electrofused two Cell Bovine Tetraploid Embryo

Purpose: The values of embryonic stem-like cell and cloning are evident. Production of cloned tissue and animals can be achieved by aggregation and injection of these cells into a tetrapolid morula and blastocyst respectively. Tetraploid embryo is used as a feeder for growth and development of stem-like cell can be produced in vitro by electrofusion of two-cell embryos. The aim of this study wa...